Document 0012
 DOCN  M9490012
 TI    [Diagnosis of FIV infection]
 DT    9411
 AU    Hartmann K; Kuffer M; Egberink H; Lutz H; Kraft W; I. Medizinischen
       Tierklinik, Ludwig-Maximilians-Universitat; Munchen.
 SO    Tierarztl Prax. 1994 Jun;22(3):268-72. Unique Identifier : AIDSLINE
       MED/94323946
 AB    The FIV PetCheck ELISA is a practical and commercially available method
       routinely used for the detection of anti-FIV antibodies in FIV
       infection. However, false positive and false negative results occur,
       although the reliability of the negative results is higher than that of
       the positive results. To determine the predictive value of a positive
       result in Bavaria, 1911 randomly selected cat sera were collected. Using
       the PetCheck anti-FIV ELISA, 103 (5.4%) of the 1911 samples tested were
       positive. These positive samples were reexamined by two other ELISA and
       by Western Blot. Sixty-one (59.2%) were positive with the second ELISA,
       53 (51.5%) with the third ELISA and 40 (40.7%) by Western Blot.
       Therefore, 59.2% of all positive samples could be considered false
       positive. The ELISA as a single method to diagnose FIV infection is not
       reliable in Germany due to the low prevalence of FIV infection.
       Therefore, a Western Blot confirmation of every positive ELISA is of
       absolute necessity.
 DE    Animal  Antibodies, Viral/*BLOOD  Blotting, Western/*VETERINARY  Cats
       English Abstract  Enzyme-Linked Immunosorbent Assay/*VETERINARY  False
       Negative Reactions  False Positive Reactions  Feline Acquired
       Immunodeficiency Syndrome/*DIAGNOSIS/EPIDEMIOLOGY  Germany/EPIDEMIOLOGY
       Immunodeficiency Virus, Feline/*IMMUNOLOGY  Predictive Value of Tests
       Prevalence  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).